C4.zip

Expression, Purification and Characterization of the Human ... - Nature

The C4 ZipTip is most commonly used in the workflow for (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight). c4.zip

: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . Expression, Purification and Characterization of the Human

: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica. : The purified protein is eluted directly onto

: The protein sample is aspirated through the tip, binding to the C4 resin.

A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons:

: Removing salts, buffers, and detergents that interfere with downstream analysis. For example, researchers use them to clean samples of Trastuzumab probes or mussel biotoxins before testing.