Because the Kuja have never seen a man, they mistake Luffy’s anatomy for a "mushroom" or an extra growth and attempt to "pull it off" while cleaning him. This leads to the shocking realization that Luffy is actually a male—a forbidden presence on their island. Key Character Introductions
A kind-hearted Kuja warrior who initially sympathizes with Luffy.
Episode 408 of , titled "Landing! No-Boys-Allowed Island Amazon Lily" , marks the start of the Amazon Lily Arc and the beginning of Luffy's solo journey following the Straw Hat crew's separation at Sabaody Archipelago. Episode Overview Manga Chapter: Adapts most of Chapter 514. Arc: Amazon Lily Arc (Saga: Summit War).
A large warrior who often repeats "The thing is..." or "Speaking of which..."
This episode transitions the series from the crushing defeat at Sabaody to a more comedic, yet high-stakes, fish-out-of-water scenario.